I would need to really think about my favourites from each film, but one that popped immediately to mind was in TUC. The scene where Qo'noS I has been attacked and is coming about, weapon ports glowing, Chekov and Valeris calling for orders, and Kirk tells Uhura to signal their surrender. Her shock and the silence on the bridge in the moment of tension, Kirk restating the order, followed by Uhura's urgent pleas with the battlecruiser, as Chekov points out that they could be facing the end.
It's one of those moments where you expect Kirk to go down firing, but goes the totally opposite direction that the reactions from everyone feels genuine, which only helps ramp up the tense moment.